DBScript Reverse Transcriptase: RT with super fast protocol

DBscript Reverse Transcriptase

(DB-1276)

DBscript Reverse Transcriptase ensures excellent cDNA synthesis from RNA templates in both one-step and two-step RT-PCR applications. With a superfast protocol and reduced pipetting steps, it features an MMLV mutant with reduced RNase H activity for improved yield and longer cDNAs (> 9 kb). It supports reverse transcription at 50-60 °C, thanks to increased thermostability and a unique buffer formulation. Expressed as a recombinant protein in E. coli, it comes with a 5X RT buffer, including dNTPs and a separate DTT solution.

Package size

Key Parameters: Superior cDNA Synthesis

Superfast Protocol: Reduced pipetting steps for streamlined workflows

Enhanced cDNA Synthesis: MMLV mutant with reduced RNase H activity for improved yield and longer cDNAs (> 9 kb)

High-Temperature Stability: Reverse transcription at 50-60 °C due to increased thermostability and unique buffer formulation

Complete Kit: Supplied with 5X RT buffer, including dNTPs and a separate DTT solution

Pricing

Name (Cat. no.)	Quantity	Price	Lead Time
DBscript Reverse Transcriptase (DB-1276)	2 kU	40 EUR	In Stock
DBscript Reverse Transcriptase (DB-1276)	10 kU	135 EUR	In Stock
DBscript Reverse Transcriptase (DB-1276)	40 kU	385 EUR	In Stock

Details

Source	Unit Definition	Storage Buffer	QC Assays
Recombinant E. coli	One unit is defined as the amount of enzyme that catalyzes incorporation of 1 nmol dNTPs (detected with SYBR® Green I dye) into 100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C.	20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol	SDS-PAGE/purity DNase, RNase, Endonuclease, DNA content Functional Assay (cDNA synthesis)

Source

Unit Definition

Storage Buffer

QC Assays

Recombinant E. coli

One unit is defined as the amount of enzyme that catalyzes incorporation of 1 nmol dNTPs (detected with SYBR® Green I dye) into

100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C.

20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol

SDS-PAGE/purity

DNase, RNase, Endonuclease, DNA content

Functional Assay (cDNA synthesis)

Downloads

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DBscript shows superior performance in more challenginig conditions

DBscript shows superior performance in more challenginig conditions:DBscript and competitor's reverse transcriptases were evaluated in 1-step qRT-PCR with equal amounts of a synthetic RNA template. Reactions were conducted in 1x RT-buffer A with 1x DTT, Bovine RNAse inhibitor (0.1 U/µl, DB-1260) and DB AptaTaq DNA Polymerase (0.2 U/µl, DB-1277). The relative cDNA yield was calculated using the equation1/2^(Ct - Ct ref) , where highest-performing sample (DBscript) served as a reference. This reflects the amount of cDNA produced relative to the reference sample. Error bars indicate standard deviation from three technical replicates.Under more challenging conditions, such as the transcription of a 2 kb template with a 10 minute reverse transcription step at 60°C, DBscript demonstrated superior performance and consistently yielded higher amounts of cDNA compared to competitor enzymes.

DBscript protocol is faster than its direct competitors

Efficient Reverse Transcription with DBscript Reverse Transcription::DBscript cDNA Synthesis Kit, powered by our thermostable DBscript Reverse Transcriptase, offers the shortest protocol time among top competitors. Its streamlined workflow accelerates primer annealing, incubation, and cDNA synthesis, without compromising performance. For researchers demanding precision and efficiency, DBscript is the optimal choice, minimizing time while maximizing output. This kit exemplifies our commitment to advancing molecular biology with faster, reliable results.

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