(DB-1285)
Unlock complete cDNA synthesis with our advanced kit, featuring a unique bovine RNase inhibitor and DBscript Reverse Transcriptase—an MMLV mutant designed for higher yield and synthesis of longer cDNAs (> 9 kb). Enjoy superior performance with reverse transcription at 50-60 °C, made possible by enhanced protein thermostability and our exclusive buffer formulation. Benefit from an ultra-fast 10-minute protocol for two-step RT-PCR and optimized buffers for seamless one-step RT-PCR.
Package size
Key Parameters: Complete cDNA synthesis with our unique bovine RNase inhibitor
DBscript Reverse Transcriptase: MMLV mutant with reduced RNase H activity for improved cDNA synthesis and increased yield and synthesis of longer cDNAs (> 9 kb).Thermus aquaticus with a half-life of over 40 minutes at 95 °C.
Reverse transcription at temperatures from 50 °C to long-term sample storage
60 °C due to a combination of increased thermostability of the protein and a unique buffer formulation.
Short protocol (10 min) for two-step RT-PCR. Optimal protocol and buffer for one-step RT-PCR. Recombinant expression in E. coli: no risk of transmission of infectious agents
DB RNase Inhibitor Bovine with exceptional oxidation resistance
Pricing
Name (Cat. no.) | Quantity | Price | Lead Time |
|---|---|---|---|
DBscript cDNA Synthesis Kit (DB-1285) | 50 rxns | 180 EUR | In Stock |
Details
Source | Content | Storage Buffer | QC Assays |
|---|---|---|---|
Recombinant E. coli | DBscript Reverse Trancriptase (DB-1276) DB RNase Inhibitor Bovine (DB-1260) Oligo(dT)20 primer, random hexamers, RT Buffer A (5X), and a separate DTT solution (20X) | 20 mM Tris 7.5 100 mM NaCl 1 mM DTT 0.1 mM EDTA 0.01 % NP-40 50 % glycerol | SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease, DNA content absence Functional Assay (cDNA synthesis) |
Downloads
Document name | Download |
|---|---|
subtitle
Related products
Reverse Transcriptase
DBscript Reverse Transcriptase is essential for the synthesis of the complementary DNA (cDNA) strand from the RNA template. Use our Reverse Transcriptase in both two-step and one-step RT-PCR to ensure excellent results in your applications.
DB Oligo(dT)20 Primer
Make your cDNA synthesis kit with our proprietary DB Oligo(dT)20 Primer.
DB Anchored Oligo(dT)20-VN Primer
Make your cDNA synthesis kit with our proprietary DB Anchored Oligo(dT)20-VN Primer.
