AF 488 Carboxylic Acid: Fluorescent Dye

(DB-410103)

AF 488 Carboxylic Acid is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 488 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.


AF 488 Carboxylic Acid is a non-reactive Alexa Fluor 488 dye used as a reference standard or for labeling peptides and proteins. Its carboxyl group can be activated (e.g., sulfo-NHS, TFP) or modified via carbodiimide chemistry for conjugation to amino groups. It’s commonly used in solid-phase peptide synthesis with activators like HATU.

Package size

Key Parameters: Great brightness and pH stability with lower costs

Carboxylic Acid: Ideal as a reference standard or for further synthesis

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 488 with friendly price.

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 488

Pricing

Cat. no.

Quantity

Price

Availibility

DB-410103-1MG

1 mg

95 EUR

In stock

DB-410103-5MG

5 mg

245 EUR

In stock

DB-410103-10MG

10 mg

360 EUR


In stock

DB-410103-25MG

25 mg

715 EUR


In stock

DB-410103-50MG

50 mg

1220 EUR


In stock

DB-410103-100MG

100 mg

1580 EUR

In stock

Shipping information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (98 %+ purity)

Optical Data of AF 488 Carboxylic Acid

Name

Excitation (nm)

Emission (nm)

Extinction coeficient (ε)

MW (g/mol)

Formula

AF-488 Carboxylic Acid

494

517

71 800

736.85

C33H44N4O11S2

AF 488 Carboxylic Acid: Absorption and emission spectra

Absorption and emission spectra of AF 488:The absorption and emission spectra of AF 488 Carboxylic Acid visualized. The absorption peak at 494 nm ensures efficient excitation, while the emission peak at 517 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 488 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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