DBdirect™ PCR Gel Mix

(DB-1272)

Introducing DBdirect™ PCR Gel Mix, a ready-to-use solution featuring blue and yellow dyes for monitoring migration during electrophoresis and an additive for direct loading onto agarose gel.


Leveraging our unique DBdirect™ technology, this mix eliminates the need for DNA extraction, streamlining your workflow for faster, more efficient results.

Package size

Key Parameters: No need for DNA isolation with faster and more streamlined workflows

End-Point PCR with aptamer hot-start. Blue and yellow dyes to monitor migration process during electrophoresis.

Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).

Suitable for single-cell detection: Certain cell types require a separate preincubation step in our Lysis Buffer A (DB-1281; feel free to request it with your order).

Possible to analyze multiple targets in one reaction.

Pricing

Cat. no.

Quantity

Price

Lead Time

DBdirect™ PCR Gel Mix (DB-1272)

100 rxns

35 EUR

In Stock

DBdirect™ PCR Gel Mix (DB-1272)

1000 rxns

276 EUR

In Stock

DBdirect™ PCR Gel Mix (DB-1272)

5000 rxns

1100 EUR

In Stock

Details

Key Protein

Hot-start

Content

QC Assays

Taq DNA Polymerase

Aptamer-based

DBdirect™ PCR Gel Mix (2x)

PCR Grade Water

Functional test (PCR)

E. coli gDNA absence

DNase Activity absence

DBdirect PCR Gel Mix: Effortless Genotyping from Mouse Tissues

Hair and Tail: Minimal Tissue input for Precise Analysis:DBdirect™ PCR Gel Mix streamlines genotyping by enabling isolation-free DNA amplification directly from mouse tail tips and hair follicles. With rapid lysis and no purification required, it saves time and resources while delivering reliable, high-sensitivity results. Perfect for high-throughput workflows, DBdirect™ ensures consistent performance with minimal tissue input!

No DNA Isolation: Sensitive PCR from Microorganisms and Cells with DBdirect Technology

Straightforward PCR Analysis with no DNA Isolation:Our DBdirect™ technology enables direct PCR from a wide range of biological samples without the need for DNA isolation. The key innovation lies in our unique PCR mix formulation, which facilitates direct lysis during the PCR reaction. Some sample types benefit from a preincubation step in DB Lysis Buffer A (DB-1281), followed by the addition of the lysate to the PCR mix. Here we compared the sensitivity of our PCR mixes to various cell types and viruses and determined the optimal DBdirect™ variant for each category. If you are using E.coli, use only 5-minute denaturation step.

Sensitivity of DBdirect™ PCR mixes across cell types and viruses

Organisms

DB-1272 with LBA

DB-1272 PCR Mix

Human Cells

+++

+++

Gram-negative bacteria (E. coli)

++

++

Gram-positive bacteria (Lactobacillus)

++

+++

Gram-positive bacteria (Rhodococcus)

++

++

Yeast (S. Cerevisiae)

++

++

Non-enveloped viruses (ADV)

+++

+++

Enveloped viruses (HSV-1)

+++

+++

+++ = 1 - 40 cells/rxn | ++ = 100 - 4000 cells/rxn | + = 10 000 - 400 000 cells/rxn

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Let's take your PCR to the next level!

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