DBdirect PCR Probe Mix (DB-1270): No need for DNA extraction

DBdirect^™ PCR Probe Mix

(DB-1270)

Discover the DBdirect™ PCR Probe Mix, a ready-to-use solution with TaqMan probes for routine and high-throughput DNA target detection.

With DBdirect™ technology, there's no need for DNA isolation, streamlining your workflow and saving valuable time. Our mixes support Real-Time PCR and allow you to analyze multiple targets in a single reaction with hot-start aptamer activation for enhanced performance.

Package size

Key Parameters: No need for NA isolation with high specificity of TaqMan Probes

Real Time PCR with Aptamer Hot-start.

Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).

Suitable for single-cell detection: Certain cell types require a separate preincubation step in our Lysis Buffer A (DB-1281; feel free to request it with your order).

Analyze multiple targets in one reaction.

Pricing

Cat. no.	Quantity	Price	Lead Time
DBdirect™ PCR Probe Mix (DB-1270)	100 rxns	35 EUR	In Stock
DBdirect™ PCR Probe Mix (DB-1270)	1000 rxns	272 EUR	In Stock
DBdirect™ PCR Probe Mix (DB-1270)	5000 rxns	1080 EUR	In Stock

Details

Key Protein	Hot-start	Content	QC Assays
Taq DNA Polymerase	Aptamer-based	DBdirect™ PCR Probe Mix (2x) PCR Grade Water	Functional test (PCR) E. coli gDNA absence DNase activity absence

Key Protein

Hot-start

Content

QC Assays

Taq DNA Polymerase

Aptamer-based

DBdirect™ PCR Probe Mix (2x)

PCR Grade Water

Functional test (PCR)

E. coli gDNA absence

DNase activity absence

No DNA Isolation: Sensitive PCR from Microorganisms and Cells with DBdirect Technology

Straightforward PCR Analysis with no DNA Isolation:Our DBdirect™ technology enables direct PCR from a wide range of biological samples without the need for DNA isolation. The key innovation lies in our unique PCR mix formulation, which facilitates direct lysis during the PCR reaction. Some sample types benefit from a preincubation step in DB Lysis Buffer A (DB-1281), followed by the addition of the lysate to the PCR mix. Here we compared the sensitivity of our PCR mixes to various cell types and viruses and determined the optimal DBdirect™ variant for each category. If you are using E.coli, use only 5-minute denaturation step.

Sensitivity of DBdirect™ PCR mixes across cell types and viruses

Organisms	DB-1270 with LBA	DB-1270 PCR Mix
Human Cells	+++	+++
Gram-negative bacteria (E. coli)	++	+++
Gram-positive bacteria (Lactobacillus)	++	+++
Gram-positive bacteria (Rhodococcus)	++	++
Yeast (S. Cerevisiae)	++	++
Non-enveloped viruses (ADV)	+++	+++
Enveloped viruses (HSV-1)	+++	+++

+++ = 1 - 40 cells/rxn | ++ = 100 - 4000 cells/rxn | + = 10 000 - 400 000 cells/rxn

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Let's take your PCR to the next level!

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