Reverse Transcription and cDNA Synthesis
Meet our complete solution for reverse transcription and cDNA synthesis applications! Our DBscript Reverse Transcriptase is essential for the synthesis of the complementary DNA (cDNA) strand from the RNA template. Try our superfast protocol for two-step RT-PCR!
Do you prefer complete solution forcDNA synthesis? DBscript cDNA Synthesis Kit is an optimized kit containing all components needed for efficient synthesis of first-strand complementary DNA (cDNA) from mRNA or total RNA templates. Use our extremely fast protocol for your prompt analysis!
We also offer separate oligos such as: DB Oligo(dT)20 primers, DB Anchored Oligo(dT)20-VN primers or DB Random Hexamers to ensure the best accuracy and efficiency for your cDNA synthesis applications.
MMLV mutant with reduced RNase H activity
Increased thermostability – up to 60 °C and transcript length 100 bp to > 9 kb
Simple protocol with minimum pippeting steps
Improved cDNA synthesis and increased yield and synthesis of longer cDNAs (> 9 kb).
Increased thermostability of the protein and a unique buffer formulation.
Optimized short protocol (1-10 min) for two-step RT-PCR
DB RNase Inhibitor Bovine with exceptional oxidation resistance
Composition: a primer sequence of 20 deoxythymidines (dTs).
Applications: cDNA synthesis, two-step RT-qPCR/RT-PCR
Composition: a primer mixture consisting of sequences of 20 deoxythymidines (dTs) followed by dV (dG, dA, or dC) and then by dN (dA, dT, dG, or dC).
Applications: cDNA synthesis, two-step RT-qPCR/RT-PCR
Composition: a primer mixture of random sequences of 6 bases [d(N)6] (N = dA, dT, dG, or dC).
Applications: cDNA synthesis, two-step RT-qPCR/RT-PCR
